The Effects of Autocrine Human Growth Hormone (hGH) on Human Mammary Carcinoma Cell Behavior Are Mediated via the hGH Receptor
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چکیده
منابع مشابه
Autocrine human growth hormone (hGH) regulation of human mammary carcinoma cell gene expression. Identification of CHOP as a mediator of hGH-stimulated human mammary carcinoma cell survival.
By use of cDNA array technology we have screened 588 genes to determine the effect of autocrine production of human growth hormone (hGH) on gene expression in human mammary carcinoma cells. We have used a previously described cellular model to study autocrine hGH function in which the hGH gene or a translation-deficient hGH gene was stably transfected into MCF-7 cells. Fifty two of the screened...
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Human growth hormone (HGH) (1, 2) has been used experimentally and therapeutically in man since 1957 (3-5). The metabolic and growth-promoting effects of the parenterally administered hormone in humans (3-5) and the antigenicity of various HGH preparations in rabbits and in guinea pigs (6, 7) are well documented. This paper describes the appearance of specific antibodiesto HGH in the plasma of ...
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In spite of recent advance in understanding of the stoichiometry of 22-kDa human GH (22K-hGH) with cell surface hGH receptor (hGHR) and hGH-binding protein (hGH-BP) circulating in human plasma, that of 20-kDa hGH (20K-hGH) is poorly understood. To clarify this, mouse pro-B Ba/F3 cells stably expressing the full-length hGHR (Ba/F3-hGHR) and both recombinant and native hGH-BP were used in this st...
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Confirmation of the diagnosis of GH deficiency in adults and children involves provocative testing for human (h) GH. Different commercially available immunoassays yield largely discrepant results in the measurement of GH levels in human serum. These discrepancies result in doubtful relevance of cut-off levels proposed for GH provocative testing. We have developed an immunofunctional assay metho...
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The 22-KDa is the protein product of human growth hormone (hGH) gene isoform 1 of the pituitary hormone (Ahmed et. al., 1993). hGH gene was amplified with PCR from human growth hormone cDNA clone by specific designed primers containing extraterminals having Nco I and Bam HI restriction sites. The PCR product obtained was analyzed by enzyme digestion and DNA sequencing. The product was cloned in...
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ژورنال
عنوان ژورنال: Endocrinology
سال: 2001
ISSN: 0013-7227
DOI: 10.1210/en.142.2.767